lentivirus rfp  (Addgene inc)

Journal: Cell Death Discovery

Article Title: Panobinostat potentiates adagrasib-induced cell death by triggering autophagy in human non-small cell lung cancer

doi: 10.1038/s41420-025-02657-9

Figure Lengend Snippet: A – C H23, Calu-1, and H1299 cells were exposed to ADA (5 μM) for specified durations, followed by Western blot to evaluate the expression of NRF2 and GAPDH. D – F H23, Calu-1, and H1299 cells were exposed to varying concentrations of ADA for 1 h, followed by Western blot to evaluate the expression of NRF2 and GAPDH. G H23 and H1299 cells were transfected with recombinant lentivirus, and the levels of NRF2 and GAPDH were subsequently measured. H H23 and H1299 cells were transfected with recombinant lentivirus. The cell survival rate was assessed after treatment with ADA (5 μM) for 24 h. I H460 cells were exposed to varying concentrations of ADA for 24 h, after which cell viability was assessed. J , K H460 cells were treated with ADA (5 μM) for specified durations, followed by Western blot to evaluate the expression of NRF2 and GAPDH. L – N H23, Calu-1, and H1299 cells were pre-incubated with DFO (100 μM) for 1 h, the cell survival rate was evaluated after treated with ADA (5 μM) for 24 h. * p < 0.05, ** p < 0.01, as determined by One-way ANOVA.

Article Snippet: The RFP-GFP-tagged LC3 lentivirus was obtained from GeneChem (Shanghai, China).

Techniques: Western Blot, Expressing, Transfection, Recombinant, Incubation

Journal: Cell Death Discovery

Article Title: Panobinostat potentiates adagrasib-induced cell death by triggering autophagy in human non-small cell lung cancer

doi: 10.1038/s41420-025-02657-9

Figure Lengend Snippet: A – C H23, Calu-1, and H1299 cells were treated with ADA (5 μM) for specified durations, followed by Western blot to assess the expression of LC3 and GAPDH. D – F H23, Calu-1, and H1299 cells were exposed to varying concentrations of ADA for 1 h, followed by Western blot to assess the expression of LC3 and GAPDH. G , H H23 and H1299 cells were transfected with lentivirus, images were captured using a fluorescence microscope after treatment with varying concentrations of ADA for 9 h. The autophagic flux can be assessed by comparing the number of GFP (green fluorescence)/mRFP (red fluorescence) double-positive vesicles. Scale bar = 25 μm.** p < 0.01, as determined by One-way ANOVA.

Article Snippet: The RFP-GFP-tagged LC3 lentivirus was obtained from GeneChem (Shanghai, China).

Techniques: Western Blot, Expressing, Transfection, Fluorescence, Microscopy

Journal: Cell Death Discovery

Article Title: Panobinostat potentiates adagrasib-induced cell death by triggering autophagy in human non-small cell lung cancer

doi: 10.1038/s41420-025-02657-9

Figure Lengend Snippet: A – C H23, Calu-1, and H1299 cells were pre-incubated with 3-MA for 1 h, the expression levels of LC3 and GAPDH were detected after treatment with ADA (5 μM) for 1 h. D , E H23 and H1299 cells were transfected with lentivirus and then pre-incubated with 3-MA for 1 h, images were captured using a fluorescence microscope after treatment with ADA (5 μM) for 9 h. F – H H23, Calu-1 and H1299 cells were pre-incubated with 3-MA for 1 h, the cell survival rate was detected after treatment with ADA (5 μM) for 24 h. I H1299 cells were pre-incubated with NAC for 1 h, the expression levels of LC3 and GAPDH were detected after treatment with ADA (5 μM) for 1 h. J H1299 cells were transfected with lentivirus and then pre-incubated with NAC for 1 h, images were captured using a fluorescence microscope after treatment with ADA (5 μM) for 9 h. Scale bar = 25 μm. * p < 0.05, ** p < 0.01, as determined by One-way ANOVA.

Article Snippet: The RFP-GFP-tagged LC3 lentivirus was obtained from GeneChem (Shanghai, China).

Techniques: Incubation, Expressing, Transfection, Fluorescence, Microscopy

Journal: Cell Death Discovery

Article Title: Panobinostat potentiates adagrasib-induced cell death by triggering autophagy in human non-small cell lung cancer

doi: 10.1038/s41420-025-02657-9

Figure Lengend Snippet: A H23 cells were treated with ADA (0.3125 μM) alone or in combination with BRU (10 nM), PAN (20 nM) and VOR (0.5 μM) for 24 h, after which cell viability was assessed. B – E H23 and H1299 cells were treated with PAN alone or in combination with various concentrations of ADA (20, 40, 80, 160, 320 nM) for 24 h, after which cell viability and related CI values were assessed. F – H H23 and H1299 cells were treated with ADA or PAN alone, or in combination for 3 h, the associated proteins were detected by Western blot. I H1299 cells were treated with ADA or PAN alone, or in combination for 9 h, the associated proteins were detected by Western blot. J , K H23 and H1299 cells were transfected with lentivirus, images were captured using a fluorescence microscope after treated with ADA or PAN alone, or in combination for 9 h. Scale bar = 25 μm. ** p < 0.01, as determined by Two-way ANOVA.

Article Snippet: The RFP-GFP-tagged LC3 lentivirus was obtained from GeneChem (Shanghai, China).

Techniques: Western Blot, Transfection, Fluorescence, Microscopy

Journal: Cell Death Discovery

Article Title: Panobinostat potentiates adagrasib-induced cell death by triggering autophagy in human non-small cell lung cancer

doi: 10.1038/s41420-025-02657-9

Figure Lengend Snippet: A H23 cells were pre-incubated with 3-MA for 1 h, the expression of LC3 and GAPDH was detected by Western blot after treated with a combination of ADA (80 nM) and PAN (20 nM) for 9 h. B , C H23 and H1299 cells were pre-incubated with 3-MA for 1 h, the cell survival rate was evaluated after treatment with ADA (80 nM for H23 cells, 160 nM for H1299 cells) and PAN (20 nM for H23 cells, 100 nM for H1299 cells) for 24 h. D , E H23 and H1299 cells were transfected with lentivirus and then pre-incubated with 3-MA for 1 h, images were captured using a fluorescence microscope after treatment with ADA (80 nM for H23 cells, 160 nM for H1299 cells) and PAN (20 nM for H23 cells, 100 nM for H1299 cells) for 9 h. F H23 cells were pre-incubated with NAC for 1 h, the expression levels of LC3 and GAPDH were detected by Western blot after treatment with ADA (80 nM) and PAN (20 nM) for 9 h. G , H H23 and H1299 cells were pre-incubated with NAC for 1 h, the cell survival rate was assessed after treatment with ADA (80 nM for H23 cells, 160 nM for H1299 cells) and PAN (20 nM for H23 cells, 100 nM for H1299 cells) for 24 h. I , J H23 and H1299 cells were transfected with lentivirus and then pre-incubated with NAC for 1 h, images were captured using a fluorescence microscope after treatment with ADA (80 nM for H23 cells, 160 nM for H1299 cells) and PAN (20 nM for H23 cells, 100 nM for H1299 cells) for 9 h. Scale bar = 25 μm. * p < 0.05, ** p < 0.01, as determined by One-way ANOVA.

Article Snippet: The RFP-GFP-tagged LC3 lentivirus was obtained from GeneChem (Shanghai, China).

Techniques: Incubation, Expressing, Western Blot, Transfection, Fluorescence, Microscopy

Journal: Cell Death Discovery

Article Title: Panobinostat potentiates adagrasib-induced cell death by triggering autophagy in human non-small cell lung cancer

doi: 10.1038/s41420-025-02657-9

Figure Lengend Snippet: A H1299 cells were subcutaneously injected into nude mice, and tumor volumes were measured in the vehicle, ADA, PAN, and combined ADA and PAN treatment groups. B An image of tumors for different groups was captured. C The weights of tumors for different groups were measured. D The body weights of nude mice in different groups were measured. E HE staining was performed on liver and kidney tissues in different groups. F Ki67 levels in the tumors were measured in different groups. G – K The expression of NRF2, LC3, p-ERK, ERK and GAPDH was evaluated by Western blot. ** p < 0.01, as determined by One-way ANOVA.

Article Snippet: The RFP-GFP-tagged LC3 lentivirus was obtained from GeneChem (Shanghai, China).

Techniques: Injection, Staining, Expressing, Western Blot